Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores
Identifieur interne : 000878 ( Main/Exploration ); précédent : 000877; suivant : 000879Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores
Auteurs : Mark E. Stearns [États-Unis] ; Lester I. Binder [Royaume-Uni]Source :
- Cell Motility and the Cytoskeleton [ 0886-1544 ] ; 1987.
English descriptors
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Abstract
We have demonstrated the presence of MAP‐2 in squirrel fish erythrophores using SDS‐PAGE, immunobolt, and immunoprecipitation techniques. The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.
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DOI: 10.1002/cm.970070305
Affiliations:
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Stearns</name><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">Philadelphie</settlement><region type="state">Pennsylvanie</region></placeName><wicri:orgArea>Department of Pharmacology, Fox Chase Cancer Center</wicri:orgArea></affiliation></author><author><name sortKey="Binder, Lester I" sort="Binder, Lester I" uniqKey="Binder L" first="Lester I." last="Binder">Lester I. Binder</name><affiliation wicri:level="3"><country>Royaume-Uni</country><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region></placeName><wicri:orgArea>Department of Anatomy, University of Alabama</wicri:orgArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Motility and the Cytoskeleton</title><title level="j" type="abbrev">Cell Motil. Cytoskeleton</title><idno type="ISSN">0886-1544</idno><idno type="eISSN">1097-0169</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1987">1987</date><biblScope unit="volume">7</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="234">234</biblScope></imprint><idno type="ISSN">0886-1544</idno></series><idno type="istex">605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7</idno><idno type="DOI">10.1002/cm.970070305</idno><idno type="ArticleID">CM970070305</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0886-1544</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>immnuolabeling</term><term>monoclonal antibodies</term><term>α‐cytomatrix</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have demonstrated the presence of MAP‐2 in squirrel fish erythrophores using SDS‐PAGE, immunobolt, and immunoprecipitation techniques. The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Midlands de l'Ouest</li><li>Pennsylvanie</li></region><settlement><li>Birmingham</li><li>Philadelphie</li></settlement></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Stearns, Mark E" sort="Stearns, Mark E" uniqKey="Stearns M" first="Mark E." last="Stearns">Mark E. Stearns</name></region></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Binder, Lester I" sort="Binder, Lester I" uniqKey="Binder L" first="Lester I." last="Binder">Lester I. Binder</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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